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Journal / Biochemistry / Horisontal gelelektroforese til forbedret…
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Biochemistry

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JoVE Journal Biochemistry
Horisontal gelelektroforese til forbedret påvisning af protein-RNA-komplekser
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Horisontal gelelektroforese til forbedret påvisning af protein-RNA-komplekser

Article DOI: 10.3791/56031-v • 06:36 min • July 28th, 2017
July 28th, 2017 •
1, 1, 1, 2, 1
1Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, 2Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School

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Native polyacrylamidgelelektroforese er et grundlæggende redskab til analyse af RNA-proteininteraktioner. Traditionelt har de fleste eksperimenter brugt vertikale geler. Imidlertid tilvejebringer vandrette geler flere fordele, såsom muligheden for at overvåge komplekser under elektroforese. Vi leverer en detaljeret protokol til generering og anvendelse af horisontal indfødte gelelektroforese.

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Biochemistry Native gel electrophoresis RNA-protein interactions mobility shift assays
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