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Journal / Biochemistry / Electroforesis en gel horizontal para la…
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JoVE Journal Biochemistry
Electroforesis en gel horizontal para la detección mejorada de los complejos proteína-ARN
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Electroforesis en gel horizontal para la detección mejorada de los complejos proteína-ARN

Article DOI: 10.3791/56031-v • 06:36 min • July 28th, 2017
July 28th, 2017 •
1, 1, 1, 2, 1
1Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, 2Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School

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La electroforesis en gel de poliacrilamida nativa es una herramienta fundamental para analizar las interacciones ARN-proteína. Tradicionalmente, la mayoría de los experimentos han utilizado geles verticales. Sin embargo, los geles horizontales proporcionan varias ventajas, tales como la oportunidad de monitorizar complejos durante la electroforesis. Proporcionamos un protocolo detallado para generar y usar electroforesis en gel nativa horizontal.

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Bioquímica Número 125 Electroforesis en gel nativo interacciones ARN-proteína ensayos de desplazamiento de movilidad
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