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Journal / Biochemistry / Ein modifiziertes Hefe-ein-Hybrid-System für…
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Biochemistry

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JoVE Journal Biochemistry
Ein modifiziertes Hefe-ein-Hybrid-System für Heteromere Protein-Komplex-DNA-Interaktionsstudien
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Ein modifiziertes Hefe-ein-Hybrid-System für Heteromere Protein-Komplex-DNA-Interaktionsstudien

Article DOI: 10.3791/56080-v • 10:47 min • July 24th, 2017
July 24th, 2017 •
1, 2, 1
1Department of Neurology, University of Southern California, 2Section of Cell and Developmental Biology, University of California San Diego

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Der hier beschriebene modifizierte Hefe-Ein-Hybrid-Assay ist eine Erweiterung des klassischen Hefe-Ein-Hybrid- (Y1H) -Assays, um die heteromere Protein-Komplex-DNA-Wechselwirkung in einem heterologen System für jede funktionelle Genomforschung zu untersuchen und zu validieren.

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Biochemie Ausgabe 125 Protein-DNA-Wechselwirkung Hefegenetik Hefe-ein-Hybrid heterologes System β-Galactosidase-Reporter Transkriptionsfaktoren Transkriptionsregulation.
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