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Journal / Cancer Research / Preparação e ensaio metabólico de 3-dimensional…
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Cancer Research

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JoVE Journal Cancer Research
Preparação e ensaio metabólico de 3-dimensional Spheroid co culturas de células de câncer no pâncreas e fibroblastos
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Preparação e ensaio metabólico de 3-dimensional Spheroid co culturas de células de câncer no pâncreas e fibroblastos

Article DOI: 10.3791/56081-v • 10:51 min • August 23rd, 2017
August 23rd, 2017 •
1, 1, 2, 2, 1, 1
1Molecular Medicine Division, Translational Genomics Research Institute, 2Agilent Technologies

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Aqui, um método é descrito para a preparação de co-cultura 3-dimensional (3D) esferoide de células de câncer no pâncreas e fibroblastos, seguidos por medição das funções metabólicas, usando um analisador de fluxo extracelular.

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Cancer Research edição 126 câncer pancreático 3-dimensional esferoides co-cultura fluxo extracelular bioenergética stress mitocondrial
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