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Journal / Genetics / Erzeugung von nativen Chromatin…
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Genetics

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JoVE Journal Genetics
Erzeugung von nativen Chromatin Immunopräzipitation Sequenzierung Bibliotheken für Nukleosom Dichteanalyse
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Erzeugung von nativen Chromatin Immunopräzipitation Sequenzierung Bibliotheken für Nukleosom Dichteanalyse

Article DOI: 10.3791/56085-v • 10:05 min • December 12th, 2017
December 12th, 2017 •
1, 1, 1, 1, 1,2
1Department of Microbiology and Immunology, Michael Smith Laboratories Centre for High-Throughput Biology, University of British Columbia, 2Canada's Michael Smith Genome Science Center, BC Cancer Agency

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Wir präsentieren eine modifizierte native Chromatin Immunopräzipitation Sequenzierung (ChIP-Seq) Methodik für die Generation der Sequenz Datasets geeignet für ein Nukleosom Dichte ChIP-Seq analytischer Rahmen micrococcal Nuklease (MNase) Zugänglichkeit zu integrieren mit Histon Modifikation Messungen.

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Genetik Ausgabe 130 Epigenomics Chromatin Immunopräzipitation Histon-Modifikation Nukleosom Dichte zelluläre Heterogenität micrococcal Nuklease
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