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Journal / Genetics / Generation av infödda kromatin…
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Genetics

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JoVE Journal Genetics
Generation av infödda kromatin Immunoprecipitation sekvensering bibliotek för Nucleosome densitet analys
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Generation av infödda kromatin Immunoprecipitation sekvensering bibliotek för Nucleosome densitet analys

Article DOI: 10.3791/56085-v • 10:05 min • December 12th, 2017
December 12th, 2017 •
1, 1, 1, 1, 1,2
1Department of Microbiology and Immunology, Michael Smith Laboratories Centre for High-Throughput Biology, University of British Columbia, 2Canada's Michael Smith Genome Science Center, BC Cancer Agency

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Vi presenterar en modifierad infödda kromatin immunoprecipitation sekvensering (ChIP-seq) metodik för generering av sekvens datamängder lämpar sig för en nukleosomens densitet ChIP-seq analytisk ram att integrera micrococcal nuclease (MNase) hjälpmedel med Histon modifiering mätningar.

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Genetik fråga 130 epigenomik kromatin immunoprecipitation Histon modifiering nukleosomens täthet cellulära heterogenitet micrococcal nuclease
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