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Journal / Developmental Biology / Horisontal helmontering: En romanbehandlings- og…
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Developmental Biology

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JoVE Journal Developmental Biology
Horisontal helmontering
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Horisontal helmontering: En romanbehandlings- og bildeprotokoll for tykke, tredimensjonale vev Tverrsnitt av huden

Article DOI: 10.3791/56106-v • 08:31 min • August 2nd, 2017
August 2nd, 2017 •
1,2, 1,2
1Centre for Stem Cells and Regenerative Medicine, King's College London, 2School of Molecular Biosciences, Washington State University

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Dette arbeidet presenterer en roman behandlings- og bildebehandling protokoll for tykk, tredimensjonal vev tverrsnitt analyse som muliggjør full utnyttelse av konfokale bildeformaliteter. Denne protokollen bevarer antigenicitet og representerer et robust system for å analysere hudhistologi og potensielt andre vevstyper.

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Utviklingsbiologi utgave 126 Tissue-tverrsnitt tredimensjonal hud hårfollikkel arrector-pili-muskel cryosectioning immunfluorescens konfokal mikroskopi
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