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JoVE Journal Developmental Biology
Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
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Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae

Article DOI: 10.3791/56121-v • 12:30 min • October 6th, 2017
October 6th, 2017 •
1, 1, 2, 1, 1
1Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, 2Reproductive Medical Centre of Guangdong Women and Children's Hospital

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Summary
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We report using an artificial intronic small RNA expression strategy to develop a non-defective recombinant Aedes aegypti densovirus (AaeDV) in vivo delivery system. A detailed procedure for the construction, packaging, and quantitative analysis of the rAaeDV vectors as well as for larval infection is described.

Tags

Recombinant Mosquito Densovirus Gene Delivery Vector Functional Analysis Of Genes Mosquito Larvae Viral-based Delivery Systems Transduction Efficiency Persistent Effects Small RNAs Target Genes Densovirus Delivery System Precursor MiRNAs MiRNA Sponges ShRNA CDNA Sequences
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