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Journal / Developmental Biology / Anvendelse af en rekombinant Mosquito Densovirus…
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Developmental Biology

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JoVE Journal Developmental Biology
Anvendelse af en rekombinant Mosquito Densovirus som en Gene levering vektor for den funktionelle analyse af gener i myg larver
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Anvendelse af en rekombinant Mosquito Densovirus som en Gene levering vektor for den funktionelle analyse af gener i myg larver

Article DOI: 10.3791/56121-v • 12:30 min • October 6th, 2017
October 6th, 2017 •
1, 1, 2, 1, 1
1Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, 2Reproductive Medical Centre of Guangdong Women and Children's Hospital

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Vi rapport ved hjælp af en kunstig intronic små RNA udtryk strategi til at udvikle en ikke-defekte rekombinant Aedes aegypti densovirus (AaeDV) i vivo levering system. En detaljeret procedure for byggeri, emballage og kvantitativ analyse af rAaeDV vektorer så godt som for larver infektion er beskrevet.

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Udviklingsmæssige biologi spørgsmålet 128 Mosquito densovirus gen levering vektor små RNA, Aedes albopictus vektor kontrol kunstige intron.
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