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Immunology and Infection

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JoVE Journal Immunology and Infection
Intratracheal Inoculation of Fischer 344 Rats with Francisella tularensis
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Intratracheal Inoculation of Fischer 344 Rats with Francisella tularensis

Article DOI: 10.3791/56123-v • 06:09 min • September 30th, 2017
September 30th, 2017 •
1, 1, 1, 2, 1, 1, 1
1South Texas Center for Emerging Infectious Diseases, University of Texas San Antonio, 2Midwestern University

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Summary
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This protocol describes intratracheal inoculations of Fischer 344 rats with Francisella tularensis. This procedure mimics pulmonary exposure of humans to this potential biothreat agent and can be used to test vaccine and therapeutic efficacy against pulmonary tularemia.

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Intratracheal Inoculation Fischer 344 Rats Francisella Tularensis Vaccine Efficacy Pulmonary Tularemia Biothreat Agent Technique Rat Lung Aerosol-generating Equipment Therapy Prevention Relevant Model Vaccine Development Pulmonary Infectious Disease Models Plague Catheter Placement Trachea Esophagus Catheter Preparation Needle Grinding Sterilization
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