JoVE Journal
Immunology and Infection
Immunology and Infection
A subscription to JoVE is required to view this content.
Chapters
Summary
Here, we describe a method that combines in situ MHC-tetramer staining with immunohistochemistry to determine localization, phenotype, and quantity of antigen-specific T cells in tissues. This protocol is used to determine the spatial and phenotypic characteristics of antigen-specific CD8 T cells relative to other cell type and structures in tissues.