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La murino colina-carenti, etionina-completati (CDE) dieta modello della lesione epatica cronica
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La murino colina-carenti, etionina-completati (CDE) dieta modello della lesione epatica cronica

Article DOI: 10.3791/56138-v • 07:28 min • October 21st, 2017
October 21st, 2017 •
1, 1, 2, 3, 1,4, 1, 5,6,7, 8,9, 10,11, 1,12
1School of Biomedical Sciences & Curtin Health Innovation Research Institute, Curtin University, 2School of Public Health & Curtin Health Innovation Research Institute, Curtin University, 3Institute of Biochemistry, Christian-Albrechts-University, 4School of Veterinary and Life Sciences, Murdoch University, 5Centenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, 6Royal Prince Alfred Hospital, 7A.W. Morrow Gastroenterology and Liver Centre, 8QIMR Berghofer Medical Research Institute, 9Faculty of Medicine and Biomedical Sciences, The University of Queensland, 10Fiona Stanley and Fremantle Hospitals, 11School of Medical and Health Sciences, Edith Cowan University, 12School of Medicine and Pharmacology, University of Western Australia

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Qui descriviamo un metodo comune per indurre la lesione cronica del fegato nei topi di alimentazione di una dieta di colina-carenti ed etionina-completati (CDE). Dimostriamo che la sorveglianza sanitaria, aspersione epatica, isolamento e conservazione. Un corso di sei settimane di tempo può informare circa il disturbo al fegato, pathohistology, fibrosi, infiammatoria e risposte delle cellule progenitrici del fegato.

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Biologia cellulare problema 128 modello murino dieta colina-carente etionina-completata l'affezione epatica cronica aspersione epatica isolamento del fegato cellule progenitrici epatiche risposta infiammatoria l'istologia fibrosi microambiente del fegato
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