JoVE Journal
Biology
Biology
This content is Open Access.
实时在体记录了拟南芥钙信号在使用荧光传感器的昆虫饲喂时
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Article
DOI:
10.3791/56142-v
•
08:21 min
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August 15th, 2017
实时在体记录了拟南芥钙信号在使用荧光传感器的昆虫饲喂时
August 15th, 2017
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Thomas R. Vincent1, James Canham1, Masatsugu Toyota2,3,4, Marieta Avramova1, Sam T. Mugford5, Simon Gilroy2, Anthony J. Miller1, Saskia Hogenhout5, Dale Sanders1
1Department of Metabolic Biology, John Innes Centre, Norwich Research Park, 2Department of Botany, University of Wisconsin, Madison, 3Department of Biochemistry and Molecular Biology, Saitama University, 4Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), 5Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
本议定书概述了喂养 hemipteran 的昆虫,如蚜虫等所产生的植物钙信号分析的简单方法。拟南芥转化与绿色荧光蛋白钙生物传感器 GCaMP3 允许实时在体成像的高时空分辨率的钙动力学。