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Sanntid i Vivo innspilling av Arabidopsis kalsium signaler under insekt fôring med et fluorescerende Biosensor
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Sanntid i Vivo innspilling av Arabidopsis kalsium signaler under insekt fôring med et fluorescerende Biosensor

Article DOI: 10.3791/56142-v • 08:21 min • August 15th, 2017
August 15th, 2017 •
1, 1, 2,3,4, 1, 5, 2, 1, 5, 1
1Department of Metabolic Biology, John Innes Centre, Norwich Research Park, 2Department of Botany, University of Wisconsin, Madison, 3Department of Biochemistry and Molecular Biology, Saitama University, 4Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), 5Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park

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Denne protokollen skisserer en enkel metode for å analysere kalsium signaler i planter generert av fôring hemipteran insekter, som bladlus. Arabidopsis thaliana forvandles med GFP kalsium biosensor GCaMP3 gir sanntid i vivo avbilding av kalsium dynamics med høy timelige og romlig oppløsning.

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Biokjemi problemet 126 kalsium bladlus GCaMP mikroskopi insekt GFP
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