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Cancer Research

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JoVE Journal Cancer Research
Prøven udvinding og samtidige kromatografiske kvantitering af Doxorubicin og Mitomycin C efter narkotika kombination levering i nanopartikler til Tumor-bærende mus
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Prøven udvinding og samtidige kromatografiske kvantitering af Doxorubicin og Mitomycin C efter narkotika kombination levering i nanopartikler til Tumor-bærende mus

Article DOI: 10.3791/56159-v • 08:57 min • October 5th, 2017
October 5th, 2017 •
1, 1, 1, 1, 1, 2, 1, 1
1Department of Pharmaceutical Sciences, University of Toronto, 2Departments of Medical Biophysics and Radiation Oncology, University of Toronto, Ontario Cancer Institute, University Health Network

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Denne protokol beskriver et effektivt og praktisk analytisk proces af prøven udvinding og samtidige bestemmelse af flere stoffer, doxorubicin (DOX), mitomycin C (MMC) og en cardio-giftige DOX metabolit, doxorubicinol (DOXol), i biologiske prøver fra en prækliniske bryst tumor model behandlet med nanopartikel formuleringer af synergistiske drug kombination.

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Kræftforskning spørgsmålet 128 HPLC simultan analyse biologisk prøve udvinding kombination kemoterapi nanopartikler farmakokinetik bio-distribution doxorubicin mitomycin C doxorubicinol blod tumor
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