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Journal / Cancer Research / Flöde flödescytometri-baserade Drug Screening…
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JoVE Journal Cancer Research
Flöde flödescytometri-baserade Drug Screening System för identifiering av små molekyler som främjar celldifferentiering av Glioblastoma stamceller
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Flöde flödescytometri-baserade Drug Screening System för identifiering av små molekyler som främjar celldifferentiering av Glioblastoma stamceller

Article DOI: 10.3791/56176-v • 10:28 min • January 10th, 2018
January 10th, 2018 •
1, 1, 2, 1,3, 1
1Department of Neurological Surgery, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, 2Department of Pathology, Johns Hopkins University, School of Medicine, 3Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine

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En effektiv screening protokoll presenteras för identifiering av små molekyler som främjar astroglial differentiering i glioblastoma stamceller (GSCs). Analysen är baserad på stamcells differentiering reporter varvid uttrycket av den förbättrade GFP (andra) drivs av mänskliga Fredsgenomförande arrangören.

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Cancerbiologi fråga 131 stamceller gliom Astrocytic differentiering Lentivirus Reporter systemet drog skärm differentiering terapi Acetylcholine Kalciumsignalering
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