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Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

Article DOI: 10.3791/56186-v • 12:47 min • August 29th, 2017
August 29th, 2017 •
1, 1, 2, 2, 1,3
1Center for Molecular Medicine Cologne (CMMC), University of Cologne, 2Cologne Center for Genomics (CCG), University of Cologne, 3Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), Research Center and Systems Biology of Ageing Cologne, University of Cologne

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Here, we describe a chromatin immunoprecipitation (ChIP) and ChIP-seq library preparation protocol to generate global epigenomic profiles from low-abundance chicken embryonic samples.

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Chromatin Immunoprecipitation ChIP Protocol Low-abundance Embryonic Samples Histone Modifications Functional Annotation Vertebrate Genomes Key Enhancers Gene Identity Limited Material Local Specific Analysis Genome-wide Analysis Transcription Regulation Cellular Heterogeneity Patent Samples Stage HH19 Chicken Embryos Albumin Extraction Extra Embryonic Membranes Removal Indian Black Ink
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