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JoVE Journal Genetics
Forberedelse af prøver og analyser af RNASeq-baseret genekspression Data fra zebrafisk
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Forberedelse af prøver og analyser af RNASeq-baseret genekspression Data fra zebrafisk

Article DOI: 10.3791/56187-v • 11:42 min • October 27th, 2017
October 27th, 2017 •
*1, *1, 1
1Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine
* These authors contributed equally

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Denne protokol præsenterer en tilgang til hele transkriptom analyse fra zebrafisk embryoner, larver, eller sorteret celler. Vi omfatter isolering af RNA, sti analyse af RNASeq data og qRT-PCR-baserede validering af gen expression ændringer.

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Genetik spørgsmålet 128 zebrafisk RNAseq transkriptom RNA Danio rerio pathway analyse Gene Ontology vilkår flow sortering fluorescens aktiveret celle sortering
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