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JoVE Journal
Biochemistry
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荧光显微镜下福尔马林固定人脑组织背景荧光的简单消除
 
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荧光显微镜下福尔马林固定人脑组织背景荧光的简单消除

Article DOI: 10.3791/56188-v 16:31 min September 3rd, 2017
September 3rd, 2017

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Summary

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生物样品的背景自发荧光常常使荧光成像技术复杂化, 特别是在老年人类 postmitotic 组织中。本协议描述了如何有效地去除这些样品的自体荧光, 利用商业上可用的发光二极管光源在免疫之前 photobleach 样品。

Tags

生物化学 问题 127 自发荧光 漂白 发光二极管 荧光显微镜 免疫荧光
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