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Journal / Biochemistry / Dissektion av mänskliga näthinnan och…
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JoVE Journal Biochemistry
Dissektion av mänskliga näthinnan och RPE-åderhinnan för proteomiska analys
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Dissektion av mänskliga näthinnan och RPE-åderhinnan för proteomiska analys

Article DOI: 10.3791/56203-v • 06:55 min • November 12th, 2017
November 12th, 2017 •
*1,2,7,8, *3,4, 3,4, 1,2, 3, 3, 1,2, 5,6, 3,9
1Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons, Columbia University, 2Edward S. Harkness Eye Institute, New York-Presbyterian Hospital, 3Omics Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, 4Medical Scientist Training Program, University of Iowa, 5Department of Pediatrics, University of Iowa, 6Department of Neurology, University of Iowa, 7Department of Ophthalmology, Federal University of Sao Paulo (UNIFESP), 8Department of Ophthalmology, Federal University of EspÍrito Santo (UFES), 9Palo Alto Veterans Administration, Palo Alto, CA
* These authors contributed equally

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Den mänskliga näthinnan består av funktionellt och molekylärt distinkta regioner, inklusive fovea, gula fläcken och perifera näthinnan. Här, beskriver vi en metod där punch biopsier och manuellt borttagande av vävnad lager från ett mänskligt öga att dissekera och samla dessa olika retinala regioner för nedströms proteomiska analys.

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Biokemi frågan 129 mänskliga näthinnan dissektion retinal pigmenterade epitel åderhinnan proteomik
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