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Journal / Genetics / Laser Microirradiation om te studeren In Vivo…
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JoVE Journal Genetics
Laser Microirradiation om te studeren In Vivo cellulaire reacties op eenvoudige en complexe DNA-schade
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Laser Microirradiation om te studeren In Vivo cellulaire reacties op eenvoudige en complexe DNA-schade

Article DOI: 10.3791/56213-v • 10:44 min • January 31st, 2018
January 31st, 2018 •
*1, *2, 2, 2, 2, 2,3,4, 1
1Department of Biological Chemistry, School of Medicine, University of California, Irvine, 2Beckman Laser Institute and Medical Clinic, University of California, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences, University of California, Irvine, 4Department of Biomedical Engineering and Surgery, University of California, Irvine
* These authors contributed equally

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Het doel van dit protocol is te beschrijven hoe laser microirradiation voor het opwekken van verschillende soorten DNA-beschadiging, met inbegrip van relatief eenvoudige strand einden en complexe schade, aan de studie van DNA schade signalering en reparatie factor montage op schade sites in vivo .

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Genetica kwestie 131 Laser microirradiation lasers in geneeskunde DNA schade DNA schade reactie DNA-reparatie poly (ADP-ribose) polymerase (PARP) complexe schade strand einden base schade
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