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Journal / Genetics / Laser Microirradiation at studere In Vivo…
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JoVE Journal Genetics
Laser Microirradiation at studere In Vivo cellulære svar til Simple og komplekse DNA-skader
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Laser Microirradiation at studere In Vivo cellulære svar til Simple og komplekse DNA-skader

Article DOI: 10.3791/56213-v • 10:44 min • January 31st, 2018
January 31st, 2018 •
*1, *2, 2, 2, 2, 2,3,4, 1
1Department of Biological Chemistry, School of Medicine, University of California, Irvine, 2Beckman Laser Institute and Medical Clinic, University of California, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences, University of California, Irvine, 4Department of Biomedical Engineering and Surgery, University of California, Irvine
* These authors contributed equally

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Målet med denne protokol er at beskrive, hvordan du bruger laser microirradiation til at fremkalde forskellige typer af DNA-skader, herunder relativt simple strand pauser og komplekse skader, at studere DNA skader signalering og reparation faktor forsamling på skade steder in vivo .

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Genetik sag 131 Laser microirradiation lasere i medicin DNA-skader DNA skader svar DNA reparation poly (ADP-ribose) polymerase (PARP) komplekse skader strand pauser base skader
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