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Journal / Genetics / Laser Microirradiation å studere i Vivo…
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Genetics

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JoVE Journal Genetics
Laser Microirradiation å studere i Vivo mobilnettet Svar å enkle og komplekse DNA skade
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Laser Microirradiation å studere i Vivo mobilnettet Svar å enkle og komplekse DNA skade

Article DOI: 10.3791/56213-v • 10:44 min • January 31st, 2018
January 31st, 2018 •
*1, *2, 2, 2, 2, 2,3,4, 1
1Department of Biological Chemistry, School of Medicine, University of California, Irvine, 2Beckman Laser Institute and Medical Clinic, University of California, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences, University of California, Irvine, 4Department of Biomedical Engineering and Surgery, University of California, Irvine
* These authors contributed equally

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Målet med denne protokollen er å beskrive hvordan du bruker laser microirradiation å indusere typer DNA-skader, inkludert relativt enkel strand bryter og komplekse skade, å studere DNA skade signalnettverk og reparasjon faktor montering på skade nettsteder i vivo .

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Genetikk problemet 131 Laser microirradiation lasere i medisin DNA skade DNA skade svar DNA-reparasjon poly (ADP-ribose) utvalg (PARP) komplekse skade strand pauser base skade
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