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Genetics

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JoVE Journal Genetics
Volgorde-specifieke en selectieve erkenning van Double-stranded RNAs over Single-stranded RNAs van chemisch gewijzigd Peptide nucleïnezuren
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Volgorde-specifieke en selectieve erkenning van Double-stranded RNAs over Single-stranded RNAs van chemisch gewijzigd Peptide nucleïnezuren

Article DOI: 10.3791/56221-v • 09:04 min • September 21st, 2017
September 21st, 2017 •
*1, *1, 1
1Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University
* These authors contributed equally

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We melden de protocollen voor de synthese en zuivering van Peptide Nucleic Acid (PNA) oligomeren opnemen gewijzigd residuen. De biochemische en Biofysische methoden voor de karakterisatie van de erkenning van RNA duplexen door de gewijzigde PNAs worden beschreven.

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Genetica kwestie 127 PNA triplex RNA structuur RNA duplex RNA-bindende liganden solid-phase peptide synthese niet-denaturering pagina 2-Aminopurine UV thermische smelten moleculaire erkenning bindende affiniteit grote groef
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