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Neuroscience

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JoVE Journal Neuroscience
Visualisering og Live bildebehandling av Oligodendrocyte Organelles i Organotypic hjernen skiver Adeno-assosiert Virus og AC Confocal mikroskopi
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Visualisering og Live bildebehandling av Oligodendrocyte Organelles i Organotypic hjernen skiver Adeno-assosiert Virus og AC Confocal mikroskopi

Article DOI: 10.3791/56237-v • 08:38 min • October 23rd, 2017
October 23rd, 2017 •
1, 1,2
1Division of Anatomy, Institute of Basic Medical Sciences, University of Oslo, 2Department of Microbiology, Oslo University Hospital

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Myelinating oligodendrocytes fremme rask action potensial forplantning og neuronal overlevelse. Beskrevet her er en protokoll for oligodendrocyte-spesifikke uttrykk for fluorescerende proteiner i organotypic hjernen skiver med påfølgende tidsinnstilt bildebehandling. Videre, en enkel prosedyre for å visualisere unstained kvinne myelin presenteres.

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Nevrovitenskap problemet 128 Time-lapse oligodendrocytes organotypic live bildebehandling mitokondrier signaltransduksjon adeno-assosiert virus myelin AC confocal refleksjon mikroskopi
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