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Bioengineering

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JoVE Journal Bioengineering
Effektiv Generation og redigering af Feeder-gratis IPSCs fra pancreas celler ved hjælp af CRISPR-Cas9 System
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Effektiv Generation og redigering af Feeder-gratis IPSCs fra pancreas celler ved hjælp af CRISPR-Cas9 System

Article DOI: 10.3791/56260-v • 09:16 min • November 8th, 2017
November 8th, 2017 •
1,2, 3, 1,2,4
1Department of Animal and Avian Sciences, University of Maryland, 2Animal Bioscience and Biotechnology Laboratory, ARS, USDA, 3NIH Stem Cell Unit, Bethesda, National Institutes of Health, 4RenOVAte Biosciences Inc

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Denne protokol beskriver i detaljer generation af fodaftryk-fri induceret pluripotente stamceller (iPSCs) fra pancreas menneskeceller i feeder-fri betingelser, efterfulgt af redigering bruger CRISPR/Cas9 ribonucleoproteins og karakterisering af den modificerede encellede kloner.

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Bioteknologi sag 129 Induced pluripotente stamceller (iPSCs) omprogrammering sendai virus CRISPR/Cas9 fodaftryk-fri iPSCs nucleofection
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