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Journal / Bioengineering / CRISPR/Cas9 복잡 한 Pronuclear를 주입 하 여 유전자 조작된 골든…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
CRISPR/Cas9 복잡 한 Pronuclear를 주입 하 여 유전자 조작된 골든 시리아 햄스터의 생산
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CRISPR/Cas9 복잡 한 Pronuclear를 주입 하 여 유전자 조작된 골든 시리아 햄스터의 생산

Article DOI: 10.3791/56263-v • 06:12 min • January 9th, 2018
January 9th, 2018 •
*1, *1,2, 1, 3, 3,4, 2,5, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 4Institute of Agriculture and Life Science, Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London
* These authors contributed equally

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클러스터의 pronuclear (PN) 주입은 정기적으로 짧은 구조 반복 (CRISPR) interspaced 그리고 CRISPR 관련 단백질-9 nuclease (CRISPR/Cas9) 시스템은 생산 하는 유전자 조작된 골든 시리아 햄스터는 매우 효율적인 방법. 여기, 우리는 유전자 녹아웃 햄스터 CRISPR/Cas9 시스템의 생산에 대 한 자세한 PN 주입 프로토콜을 설명합니다.

Tags

생명 공학 문제 131 Pronuclear 주입 CRISPR/Cas9 진 편집 녹아웃 zygote 배아 전송 동물 모델 시리아 황금 햄스터
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