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Journal / Developmental Biology / Hurtig, instrueret differentiering af retinale…
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Developmental Biology

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JoVE Journal Developmental Biology
Hurtig, instrueret differentiering af retinale Pigment epitel celler fra menneskelige embryonale eller induceret pluripotente stamceller
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Hurtig, instrueret differentiering af retinale Pigment epitel celler fra menneskelige embryonale eller induceret pluripotente stamceller

Article DOI: 10.3791/56274-v • 10:48 min • October 30th, 2017
October 30th, 2017 •
1, 1
1Center for Stem Cell Biology and Engineering, University of California, Santa Barbara

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Denne protokol beskriver hvordan man producerer retinale pigment epitel celler (ÅV) fra pluripotente stamceller. Metoden bruger en kombination af vækstfaktorer og små molekyler for at styre differentieringen af stamceller til umodne ÅV i 14 dage og modne, funktionelle ÅV efter tre måneder.

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Udviklingsmæssige biologi spørgsmålet 128 retinale pigment epitel celler (ÅV) retinale pigment epitel inducerede pluripotente stamceller humane embryonale stamceller differentiering retinal udvikling cellekultur
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