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JoVE Journal Genetics
Microinjection de protéine CRISPR/Cas9 dans des embryons de barbue de rivière, Ictalurus punctatus, pour Gene Editing
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Microinjection de protéine CRISPR/Cas9 dans des embryons de barbue de rivière, Ictalurus punctatus, pour Gene Editing

Article DOI: 10.3791/56275-v • 16:08 min • January 20th, 2018
January 20th, 2018 •
*1,2, *1,3, 1, 4, 4, 5, 5, 1
1School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, 4Department of Molecular Physiology and Biophysics, Vanderbilt University, 5Life Science Institute, University of Michigan
* These authors contributed equally

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Un protocole de microinjection simple et efficace pour l’édition de gène dans des embryons de barbue en utilisant le système CRISPR/Cas9 est présenté. À ce protocole, le guide des ARN et protéine Cas9 étaient micro dans le jaune d’embryons unicellulaire. Ce protocole a été validé par assommant deux gènes immunitaires de barbue.

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Genetics numéro 131 CRISPR/Cas9 gène édition Knock-out du gène micro-injection embryons de barbue mutation tronqué de protéine indels
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