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Neuroscience

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JoVE Journal Neuroscience
Seguita dalla singola cellula di rilevamento a Monitor biologia cellulare e la progressione di lignaggio di popolazioni neurali Multiple immagini dal vivo
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Seguita dalla singola cellula di rilevamento a Monitor biologia cellulare e la progressione di lignaggio di popolazioni neurali Multiple immagini dal vivo

Article DOI: 10.3791/56291-v • 10:55 min • December 16th, 2017
December 16th, 2017 •
*1,2,3, *1,2,3, *4,5, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 6, 7, 1,2,3, 1,2,3
1Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University, 2University Institute for Neurochemistry Research (IUIN), 3Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 4Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilians University Munich, 5Toxicology and Pharmacology Department, Faculty of Veterinary medicine, Complutense University, 6Brain Institute, Federal University of Rio Grande do Norte, 7Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich
* These authors contributed equally

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È descritto un protocollo robusto per monitorare le popolazioni neurali di time-lapse video-microscopia seguita da basati su software di post-elaborazione. Questo metodo rappresenta un potente strumento per identificare gli eventi biologici in una popolazione selezionata durante esperimenti dal vivo di formazione immagine.

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Neuroscienze problema 130 Live imaging singola cella di rilevamento progressione di lignaggio cellule staminali neurali adulte cellule neurali albero di lignaggio time-lapse video-microscopia
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