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Journal / Neuroscience / Live Imaging följt av enstaka Cell spårning…
JoVE Journal
Neuroscience

This content is Open Access.

JoVE Journal Neuroscience
Live Imaging följt av enstaka Cell spårning Monitor cellbiologi och härstamning progressionen av flera neurala populationer
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Live Imaging följt av enstaka Cell spårning Monitor cellbiologi och härstamning progressionen av flera neurala populationer

Article DOI: 10.3791/56291-v • 10:55 min • December 16th, 2017
December 16th, 2017 •
*1,2,3, *1,2,3, *4,5, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 6, 7, 1,2,3, 1,2,3
1Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University, 2University Institute for Neurochemistry Research (IUIN), 3Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 4Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilians University Munich, 5Toxicology and Pharmacology Department, Faculty of Veterinary medicine, Complutense University, 6Brain Institute, Federal University of Rio Grande do Norte, 7Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich
* These authors contributed equally

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Ett robust protokoll att övervaka neurala populationer av time-lapse video-mikroskopi följt av mjukvaran-baserat efterbehandling beskrivs. Denna metod utgör ett kraftfullt verktyg för att identifiera biologiska händelser i en vald population under levande imaging experiment.

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Neurovetenskap fråga 130 levande imaging enda cell tracking härstamning progression vuxna neurala stamceller neurala celler härstamning träd time-lapse video-mikroskopi
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