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Journal / Neuroscience / Imaging Neural Activity in the Primary…
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Neuroscience

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JoVE Journal Neuroscience
Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice
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Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Article DOI: 10.3791/56297-v • 07:04 min • January 7th, 2019
January 7th, 2019 •
1,2, 3, 1, 4, 1, 1
1Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Department of Anatomy and Cell Biology, Indiana University School of Medicine, 2Department of Spinal Cord Injury and Repair, Trauma and Orthopedics Institute of Chinese PLA, General Hospital of Jinan Military Region, 3Department of Orthopedics, Shandong Cancer Hospital, Shandong University, 4Department of Neurobiology, Institute of Basic Medical Sciences, Academy Military Medical Sciences

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Summary
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We describe an experimental procedure for measuring neuronal activity through dual optical windows above bilateral primary somatosensory corticies (S1) in Thy1-GCaMP6s transgenic mice using 2-photon (2P) microscopy in vivo.

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Neural Activity Primary Somatosensory Cortex Thy1-GCaMP6s Transgenic Mice Two Photon Technique Optical Windows Warming Incubation Toe Pinch Response Anesthetized Mouse Head-holding Adapter Scalp Shaving Sterile Alcohol Preparation Pads Povidone-iodine Solution Scrubs Exposure Area
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