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Journal / Immunology and Infection / Dirigida a tioles de la cisteína para in Vitro…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Dirigida a tioles de la cisteína para in Vitro específica glicosilación de proteínas recombinantes
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Dirigida a tioles de la cisteína para in Vitro específica glicosilación de proteínas recombinantes

Article DOI: 10.3791/56302-v • 11:25 min • October 4th, 2017
October 4th, 2017 •
*1, *1, *1, *1, 1, 1
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario
* These authors contributed equally

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Análisis bioquímicos y estructurales de proteínas glicosiladas requieren cantidades relativamente grandes de muestras homogéneas. Aquí, presentamos un método químico eficiente para el glycosylation sitio-específico de proteínas recombinantes purificados de bacterias dirigiéndose a tioles reactivos de Cys.

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Inmunología número 128 glicosilación methanethiosulfonate modificación de tiol de cisteína interacción stromal molecule-1 EFSAM espectroscopia de resonancia magnética nuclear de la solución espectrometría de masas
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