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Journal / Genetics / Immunostaining para modificaciones de ADN:…
JoVE Journal
Genetics

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JoVE Journal Genetics
Immunostaining para modificaciones de ADN
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Immunostaining para modificaciones de ADN: análisis computacional de imágenes confocales

Article DOI: 10.3791/56318-v • 09:42 min • September 7th, 2017
September 7th, 2017 •
*1, *1, 1, 1, 1, 2, 2, 1, 3, 1
1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, 3Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham
* These authors contributed equally

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Recién descubierto formas oxidadas de la 5-METILCITOSINA (oxi-mCs), 5-hidroximetilcitosina (5hmC), 5-formylcytosine (fC 5) y 5-carboxylcytosine (5caC) puede representar distintas modificaciones de ADN con papeles funcionales únicas. Aquí se describe un flujo de trabajo semi cuantitativa para la visualización de la distribución espacial de la oxi-mCs, perfiles de intensidad de señal y colocalización.

Tags

Genética número 127 epigenética ADN oxi-mCs N6-methyladenine Immunohistochemistry Zen microscopia Confocal señal de cuantificación distribución espacial
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