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Journal / Genetics / Immunostaining per modificazioni del DNA: analisi …
JoVE Journal
Genetics

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JoVE Journal Genetics
Immunostaining per modificazioni del DNA
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Immunostaining per modificazioni del DNA: analisi computazionale delle immagini Confocal

Article DOI: 10.3791/56318-v • 09:42 min • September 7th, 2017
September 7th, 2017 •
*1, *1, 1, 1, 1, 2, 2, 1, 3, 1
1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, 3Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham
* These authors contributed equally

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Recentemente scoperto forme ossidate di 5-metilcitosina (oxi-mCs), 5-idrossimetilcitosina (5hmC), 5-formylcytosine (5fC) e 5-carboxylcytosine (5caC) può rappresentare distinte modificazioni del DNA con ruoli funzionali unici. Qui è descritto un flusso di lavoro semi-quantitativa per visualizzazione della distribuzione spaziale di oxi-mCs', profilatura di intensità di segnale e colocalizzazione.

Tags

Problema 127 epigenetica DNA genetica oxi-mCs N6-methyladenine Immunohistochemistry Zen microscopia confocale quantificazione di segnale distribuzione spaziale
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