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Journal / Genetics / 无基因克隆的基因破坏的变形链球菌菌株的生成…
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Genetics

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JoVE Journal Genetics
无基因克隆的基因破坏的变形链球菌菌株的生成
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无基因克隆的基因破坏的变形链球菌菌株的生成

Article DOI: 10.3791/56319-v • 12:06 min • October 23rd, 2017
October 23rd, 2017 •
1, 1, 1,2,3, 1
1Department of Translational Research, Tsurumi University School of Dental Medicine, 2Endowed Department of International Oral Health Science (affiliated with Department of Translational Research), Tsurumi University School of Dental Medicine, 3Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University

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我们描述了一种简便, 快速, 和相对廉价的方法, 产生基因中断的变形链球菌菌株;这种技术可以适应于各种物种的基因中断菌株的产生。

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遗传学 问题 128 基因中断 DNA 结构 聚合酶链反应 引物设计 抗生素抗性标记基因,变形链球菌 电穿孔 同源重组
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