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Journal / Bioengineering / Organoid rekonstitution Assay (ORA) for den…
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Bioengineering

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JoVE Journal Bioengineering
Organoid rekonstitution Assay (ORA) for den funktionelle analyse af Intestinal stilk og Niche celler
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Organoid rekonstitution Assay (ORA) for den funktionelle analyse af Intestinal stilk og Niche celler

Article DOI: 10.3791/56329-v • 09:38 min • November 20th, 2017
November 20th, 2017 •
1, 1, 1, 1
1Department of Pathology, Erasmus MC Cancer Institute, Erasmus University Medical Center

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Tarm organoid kulturer er etableret fra hele krypter og tillader ikke, at analysen af selvfornyelse og differentiering i en celle-specifikke mode. Denne protokol beskriver rekonstituering af sorterede stilk (Lgr5+) og niche (Paneth) celler, som giver anledning til organoids samtidig med at deres forudgående biokemiske og genetiske modifikation og funktionelle analyse.

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Bioteknologi sag 129 mini gut organoids Lgr5+ stamceller celler Paneth cellerne intestinal stamcelle niche organoid rekonstitution assay intestinal Co kultur
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