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Betinget Knockdown av genuttrykk i kreftcelle linjer å studere rekruttering av monocytter/makrofager til Tumor Microenvironment
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Betinget Knockdown av genuttrykk i kreftcelle linjer å studere rekruttering av monocytter/makrofager til Tumor Microenvironment

Article DOI: 10.3791/56333-v • 10:59 min • November 23rd, 2017
November 23rd, 2017 •
1,2, 1,2,3
1Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics, University of Southern California, 2The Saban Research Institute of Children's Hospital Los Angeles, 3Department of Biochemistry and Molecular Medicine, University of Southern California

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Denne protokollen fungerer som en ordning for å sette opp en funksjonell Tet-ON-system i kreftcelle linjer og dets senere bruk, spesielt for å studere rollen til svulst celle-avledet proteiner i rekrutteringen av monocytter/makrofager til tumor microenvironment.

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Kreftforskning problemet 129 betinget banke ned celle migrasjon doxycycline shRNA PAI-1 monocytter kreftcelle linjer
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