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Divisão celular dos mamíferos em matrizes 3D através de microscopia de reflexão Confocal quantitativa
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Divisão celular dos mamíferos em matrizes 3D através de microscopia de reflexão Confocal quantitativa

Article DOI: 10.3791/56364-v • 10:22 min • November 29th, 2017
November 29th, 2017 •
*1,2, *1, 1, 1, 3, 1,2, 3, 1,2,4
1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
* These authors contributed equally

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Este protocolo eficientemente estuda divisão de células de mamíferos em matrizes de colágeno 3D, integrando a sincronização da divisão celular, monitoramento de eventos de divisão em matrizes 3D usando a técnica de imagem live-célula, microscopia de reflexão confocal tempo-resolvido e análise quantitativa de imagem.

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Bioengenharia edição 129 divisão celular Matrix 3D sincronização interação célula-matriz microscopia Confocal reflexão
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