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Bioengineering

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JoVE Journal Bioengineering
Zoogdieren celdeling in 3D-Matrices via reflectie van kwantitatieve confocale microscopie
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Zoogdieren celdeling in 3D-Matrices via reflectie van kwantitatieve confocale microscopie

Article DOI: 10.3791/56364-v • 10:22 min • November 29th, 2017
November 29th, 2017 •
*1,2, *1, 1, 1, 3, 1,2, 3, 1,2,4
1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
* These authors contributed equally

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Dit protocol bestudeert efficiënt zoogdieren celdeling in 3D collageen matrices door de integratie van de synchronisatie van de celdeling, toezicht op divisie gebeurtenissen in 3D-matrices met behulp van live-cel beeldvormende techniek, time-resolved confocal reflectie microscopie en kwantitatieve analyse van beeldvorming.

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Bioengineering kwestie 129 celdeling 3D Matrix synchronisatie cel-Matrix interactie reflectie van de Confocal Microscopie
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