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Journal / Biochemistry / En alternativ kultur metode til at opretholde…
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Biochemistry

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JoVE Journal Biochemistry
En alternativ kultur metode til at opretholde genomisk Hypomethylation af mus embryonale stamceller ved hjælp af MEK hæmmer PD0325901 og C-Vitamin
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En alternativ kultur metode til at opretholde genomisk Hypomethylation af mus embryonale stamceller ved hjælp af MEK hæmmer PD0325901 og C-Vitamin

Article DOI: 10.3791/56391-v • 11:53 min • June 1st, 2018
June 1st, 2018 •
1, 1, 1
1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences

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Vi beskrev i detaljer to kemiske-baserede protokoller for dyrkning mus embryonale stamceller. Denne nye metode udnytter synergistisk mekanismer til at fremme Tet-medierede oxidation (af C-vitamin) og undertrykke de novo syntesen af 5-methylcytosine (af PD0325901) for at opretholde DNA hypomethylation og pluripotency mus embryonale stamceller.

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Biokemi sag 136 C-Vitamin PD0325901 mus embryonale stamceller-celler DNA hypomethylation Prdm14 pluripotency
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