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Journal / Genetics / Em pool shRNA tela para reativação de MeCP2 no…
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Em pool shRNA tela para reativação de MeCP2 no cromossomo X inativo
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Em pool shRNA tela para reativação de MeCP2 no cromossomo X inativo

Article DOI: 10.3791/56398-v • 11:15 min • March 2nd, 2018
March 2nd, 2018 •
1,2, 1, 1, 1, 1, 1, 1, 3, 1,4
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry, University of Washington

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Nós relatamos um gancho de cabelo pequeno RNA (shRNA) e a próxima geração baseada no sequenciamento protocolo identificando os reguladores da inativação do cromossomo x em uma linha de celular murino com luciferase de vaga-lume e genes de resistência de hptII fundiram para o metil CpG ligação proteína 2 ( MeCP2) Gene no cromossomo X inativo.

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Genética epigenética edição 133 inativação do cromossomo x MeCP2 shRNA XIST síndrome de Rett triagem
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