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Genetics

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在非活动 X 染色体上重新激活 MeCP2 的池 shRNA 屏幕
 
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在非活动 X 染色体上重新激活 MeCP2 的池 shRNA 屏幕

Article DOI: 10.3791/56398-v 11:15 min March 2nd, 2018
March 2nd, 2018

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Summary

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我们报告一个小发夹 RNA (shRNA) 和下一代测序协议, 以确定在小鼠细胞系的 X 染色体失活的监管者与萤火虫荧光素酶和潮霉素抵抗基因融合到甲基 CpG 结合蛋白 2 (MeCP2)非活性 X 染色体上的基因。

Tags

遗传学 问题 133 X 染色体失活 表观遗传学 MeCP2 Rett 综合征 XIST shRNA 筛查
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