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Journal / Genetics / Gepoolte ShRNA Bildschirm für die Reaktivierung…
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Genetics

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JoVE Journal Genetics
Gepoolte ShRNA Bildschirm für die Reaktivierung des MeCP2 auf dem inaktiven X-Chromosom
Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)
Journal (Genetics)
Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)
Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
Journal (Genetics)
Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
 
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Gepoolte ShRNA Bildschirm für die Reaktivierung des MeCP2 auf dem inaktiven X-Chromosom

Article DOI: 10.3791/56398-v • 11:15 min • March 2nd, 2018
March 2nd, 2018 •
1,2, 1, 1, 1, 1, 1, 1, 3, 1,4
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry, University of Washington

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Wir berichten über eine kleine Hairpin RNA (ShRNA) und die nächste Generation Sequencing-basiertes Protokoll zur Identifizierung von Regulatoren der X-Chromosom Inaktivierung in einem murinen Zelllinie mit Firefly Luciferase und Hygromycin-Resistenz-Gene verschmolzen, das Methyl CpG verbindliches Protein 2) MeCP2) gen auf dem x-Chromosom inaktiv.

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Ausgabe 133 X-Chromosom Inaktivierung Genetik Epigenetik MeCP2 Rett-Syndrom XIST ShRNA screening
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