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Journal / Genetics / Havuza alınan shRNA MeCP2 reaktivasyonu üzerinde…
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Genetics

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JoVE Journal Genetics
Havuza alınan shRNA MeCP2 reaktivasyonu üzerinde etkin olmayan X kromozomu için ekran
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Havuza alınan shRNA MeCP2 reaktivasyonu üzerinde etkin olmayan X kromozomu için ekran

Article DOI: 10.3791/56398-v • 11:15 min • March 2nd, 2018
March 2nd, 2018 •
1,2, 1, 1, 1, 1, 1, 1, 3, 1,4
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry, University of Washington

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Biz bir küçük saç tokası RNA (shRNA) ve düzenleyiciler, x kromozomu inactivation ateş böceği luciferase ile bir fare hücre satırı tanımlamak için sonraki nesil sıralama tabanlı iletişim kuralı raporu ve hygromycin direnç genleri metil için erimiş CpG bağlayıcı protein 2) MeCP2) etkin olmayan X kromozomu üzerindeki gen.

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Genetik epigenetik sayı: 133 x kromozomu inactivation MeCP2 Rett sendromu XIST shRNA eleme
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