Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Chip-based Three-dimensional Cell Culture in…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
Journal (Biology)
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
Supported Planar Bilayers for the Formation of Study of Immunological Synapses and Kinapse
Journal (Biology)
Supported Planar Bilayers for the Formation of Study of Immunological Synapses and Kinapse
 
Click here for the English version

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors

Article DOI: 10.3791/564-v • 12:40 min • May 21st, 2008
May 21st, 2008 •
1, 1, 1, 1, 1, 1
1Institute for Biological Interfaces, Forschungszentrum Karlsruhe

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

We describe a chip-based platform for the three-dimensional cultivation of cells in micro-bioreactors. One chip can house up to 10 Mio. cells that can be cultivated under precisely defined conditions with regard to fluid flow, oxygen tension etc. in a sterile, closed circulation loop.

Tags

Chip-based Three-dimensional Cell Culture Perfused Micro-bioreactors Chip Non-biodegradable Polymers Bio-degradable Polymers Dimensions Cubic Micro-containers Round Recesses Scaffold Cells Nutrient Supply Gas Supply Bioreactor Housing Closed Steril Circulation Loop Roller Pump Medium Reservoir Perfusion Superfusion Mixed Operation Mode Cell Lines Primary Cells Organotypic Functions Hepatocellular Carcinoma Cell Lines Liver Specific Genes
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter