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Journal / Neuroscience / Imágenes en vivo de la proteína GLUT4 trata de…
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Neuroscience

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JoVE Journal Neuroscience
Imágenes en vivo de la proteína GLUT4 trata de ratón las neuronas hipotalámica primaria utilizando microscopía de deconvolución
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Imágenes en vivo de la proteína GLUT4 trata de ratón las neuronas hipotalámica primaria utilizando microscopía de deconvolución

Article DOI: 10.3791/56409-v • 08:47 min • December 7th, 2017
December 7th, 2017 •
1,2,3, 4,5, 4,5,6
1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine, Taipei Medical University, 3Core Facility, Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology, Taipei Medical University

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Este protocolo describe una técnica para la observación de la fluorescencia verde en tiempo real la proteína (GFP) etiquetado proteína del transportador de glucosa 4 (GLUT4) trata sobre la estimulación de la insulina y la caracterización de la función biológica de CCR5 en la insulina, GLUT4 señalización vía con microscopía de deconvolución.

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Neurobiología número 130 ratón primaria neurona hipotalámica cultura grabación de imágenes en vivo transfection de la liposoma basado GLUT4 deconvolución en microscopía
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