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JoVE Journal Genetics
Determinação das taxas de decaimento transcrição de todo o genoma em quiescentes e proliferação de fibroblastos humanos
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Determinação das taxas de decaimento transcrição de todo o genoma em quiescentes e proliferação de fibroblastos humanos

Article DOI: 10.3791/56423-v • 07:03 min • January 2nd, 2018
January 2nd, 2018 •
1,2, 1,2,3, 1,2,3
1Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, 2Department of Biological Chemistry, David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program, University of California, Los Angeles

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Descreveremos um protocolo para gerar proliferando e quiescentes fibroblastos dérmicos humanos primários, taxas de decomposição de transcrição de monitoramento e identificação de genes diferencialmente em decomposição.

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Genética questão 131 deterioração de transcrição quiescência fibroblastos inibição de contato Half-Life actinomicina D miR-29
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