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Journal / Immunology and Infection / Overexpressie en zuivering van het menselijk
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Immunology and Infection

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JoVE Journal Immunology and Infection
Overexpressie en zuivering van het menselijk
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Overexpressie en zuivering van het menselijk

Article DOI: 10.3791/56430-v • 07:35 min • August 3rd, 2017
August 3rd, 2017 •
*1, *1, 2, 2, 1,3, 1,3, 2, 2,4, 2
1Sackler Faculty of Medicine, Tel Aviv University, 2Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 3Department of Ophthalmology, Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University, 4Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University
* These authors contributed equally

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Een eenvoudig protocol voor overexpressie en zuivering van codon-geoptimaliseerde, humane cis -prenyltransferase, onder niet-denaturerende omstandigheden, van Escherichia Coli , wordt beschreven, samen met een enzymatische activiteitstest. Dit protocol kan worden genegaliseerd voor de productie van andere cis- prenyltransferase eiwitten in hoeveelheid en kwaliteit geschikt voor mechanistische studies.

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Immunologie Probleem 126 codonoptimalisatie heterologe overexpressie eiwitzuivering prenyltransferase dehydrodolichyldifosfaatsynthase polyprenyl
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