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JoVE Journal
Immunology and Infection

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人类的过表达和纯化
 
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人类的过表达和纯化

Article DOI: 10.3791/56430-v 07:35 min August 3rd, 2017
August 3rd, 2017

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

来自大肠杆菌的非变性条件下用于过表达和纯化密码子优化的人顺式 -丙烯基转移酶的简单方案 大肠杆菌 ,以及酶活性测定法。该方案可以推广用于生产适合于机械研究的数量和质量的其它顺式异戊烯基转移酶蛋白质。

Tags

免疫学,第126期,密码子优化,异源过表达,蛋白纯化,异戊烯基转移酶,脱氢二酰基二磷酸合酶,聚异戊二烯
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